EvolutionEcology Essay Example | Topics and Well Written Essays - 500 words

EvolutionEcology - Essay Example Paper starts with the hypothesis demonstrating mitochondrial DNA as primary tool for investigation of evolutionary diversion among closely related species and development of new approach in terms of PCR-RFLP based rapid and inexpensive techniques to established phylogenitic correlation among different species. They also investigated correlation between two different approaches and indicated that data obtained by two different techniques may not be identical and hence caution must be taken to interpret them. For validation of hypothesis the authors selected four avian sp. found in North America, those having high rate of hybridization namely, Dendroica occidentalis, D. townsendi, D. virens, and D.nigrescens. To investigate above mention hypothesis the first experiment was based on classical RFLP based technique. The total mitochondrial DNA were Isolated and digested with 14 restriction enzymes to obtained band pattern which was subsequently analyzed by David L. Swofford's paup* 4.0d64 program for calculation of Nei-Li distances. Similarly, for sequences based analysis, three genes located on two sites on mtDNA were selected and sequenced, namely 681bp cytochrome oxidase I, and 1074bp ATP synthase8 and 6 genes from 30 representative warbler individuals.